Creative Biogene Introduces the Latest Luciferase

For research and investigation in the small scale universe of cells and qualities, hereditary columnist cell is an extremely helpful device for perception and measurement, with an extensive variety of utilizations.

Shirley, USA – November 17, 2016 /MarketersMedia/ —

For research and exploration in the micro world of cells and genes, genetic reporter cell is a very useful tool for visualization and quantification, with a wide range of applications. Luciferase cell can become the ideal report gene through quantitative detection with excellent sensitivity.

Now, Creative Biogene will introduce several new luciferase.

Gaussia luciferase is a secretory blue luciferase with smaller protein molecular weight at only 22KD. It contains a natural secretory signal peptide, which can guide the luciferase secreted into cell-cultured supernatant, so as to detect reporter gene activity without cell lysis. More than 85% of the expressed luciferase are secreted into the extracellular area. But because of the secreted luciferase signal is many times stronger than that from Firefly or Renilla, residual luciferase can be detected in cell lysates. Secretory expression brings great convenience for the detection without cracking precious cells to analyze real-time dynamic living cells.

Cypridina luciferase is a secreted luciferase in beautiful blue purple with protein molecular weight of 62KD. Like Gaussia, most of the luciferase products can be secreted into the extracellular area, which can be used for continuous detection of living cells. Because the signal is very strong, the remaining cells in the luciferase is sufficient for routine detection of cell lysis, and multiple detection can be achieved. Cypridina (sea firefly) luciferase and red firefly luciferase can be combined for double color detection.

Red Firefly luciferase is an intracellular protein, whose emission peak is migrated to the red spectral region, compared with the natural Firefly luciferase (green). It is really good news” for luciferase of “flourishing leaves and withering flowers”. It is because of the spectral migration of Red Firefly luciferase, it can separate Gaussia, Cypridina or Green Renilla luciferase to be used in combination.

Beetle luciferase can share the same substrate. They have the same size and most of the amino acids. It is a good choice for dual color detection.

Green Renilla luciferase is an intracellular protein. Compared with the natural Renilla luciferase, it has the higher stability in serum, and the luminescence intensity is better. Therefore, the use of Green Renilla luciferase can get higher reporter gene detection sensitivity.

The same as the ideal reporter gene fluorescent protein, GFP cell now already has a colorful world. Then what are the characteristics of fluorescent protein and luciferase? Fluorescent protein can not get self-luminescence, but requires an excitation light source before it can generate emission fluorescence. Its luminescence energy is from the excited light. The advantages of fluorescent protein do not require substrate and interferential cell for continuous detection. However, the excitatory light can cause background fluorescence.

Single luciferase assay selection is quite rich. Researchers can combine different luciferase for multiple detection according to the needs of study.

For more information, please visit http://www.creative-biogene.com/

Contact Info:
Name: Wendy Wilson
Organization: Creative Biogene
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Phone: 5166698109

Source: http://marketersmedia.com/creative-biogene-introduces-the-latest-luciferase/147087

Release ID: 147087